Fig. 3
MLN8237 inhibited tumor growth in mice model. A total of 5 × 106 of IMR32 cells were subcutaneously inoculated in the right flank of each BALB/c nude mice. When the largest tumor reached about 100 mm3, the mice were randomly divided into four groups and given MLN8237 (30 mg/kg, 15 mg/kg, 5 mg/kg, and 0 mg/kg, respectively) once daily by oral gavage. a–c When tumors reached a size of 1.8 cm diameter, mice were sacrificed. Tumor volume (before and after treatment), tumor weight, and mice body weight were analyzed among the different group. d IHC staining was performed to analyse phospholyrated H3 and methylated H3K9