Fig. 7

MLN8237 treatment led to abnormal high expression of AURKA in vivo. a At the end of the treatment, the tumor tissues were removed from the transplanted tumor model of mice and made into paraffin sections. The tissue slides derived from mice model treated with 5 mg/kg of MLN8237, 15 mg/kg of MLN8237, 30 mg/kg of MLN8237, respectively. IHC staining was performed to analyse AURKA expression. b Extraction of total protein from tumor tissues and detection of AURKA expression by western blot. The level of total AURKA was compared between 30 mg/kg treatment group, 15 mg/kg treatment group c, and control group, respectively. d The tumor sizes of each mouse in 30 mg/kg of MLN8237 group were monitored during the period of treatment