Fig. 9

MLN8237 treatment followed by knockdown of AURKA forced senescent cells into apoptosis. a IMR32 cells were treated with 2 μmol/l of MLN8237. Two hours later, MLN8237 withdrawal followed by siAURKA-1 transfection. At 24-h after transfection, removing the supernatant, cells were cultured in normal medium in the presence of 2 μmol/l of MLN8237 till 72 h. Cellular senescence was evaluated by SA-β-gal staining, cell apoptosis (b) was assessed by flow cytometry. Cell viability (c) was assayed by MTT method from day 1 to day 6 after 2 μmol/l of MLN8237 treatment, or siAURKA transfection alone, or MLN8237 treatment plus siAURKA transfection, or no treatment as control. Each sample was analyzed by triplicates. Error bars correspond to the averages ± S.D. d IMR32 cells were treated with 2 μmol/l of MLN8237. At 48 h after transfection, cells were harvested and total protein were isolated. Western blots were assayed for pAkt, pSTAT3, and Bmi1. Gray values were calculated by imageJ software. The data were shown as the mean ± SEM of three independent experiments. *P < 0.05