Fig. 4

LINC00908 elevates the expression of TSPYL5 by binding to miR-483-5p. a TSPYL5 screened out using TargetScan (http://www.targetscan.org/vert_71/), miRDB (http://mirdb.org/miRDB/index.html), and mirwalk (http://mirwalk.umm.uni-heidelberg.de/) databases, in combination with the microarray GSE38241. The four ellipses plotted in the figure represent the predicted results from the TargetScan, database, miRDB and mirwalk databases as well as GSE38241 dataset respectively. The middle part represents the intersecting genes from them. b The luciferase activity of Wt-TSPYL5 or Mut-TSPYL5 in cells transfected with miR-483-5p mimic. *p < 0.05 vs. cells upon mimic NC treatment. c The expression of TSPYL5 in VCaP, LNCaP, DU-145, and PC-3 cells and normal RWPE-1 cell line detected by Western blot analysis, normalized to β-actin, * p < 0.05 vs. RWPE-1 cells. d The protein expression of TSPYL5 in LNCaP cells after the alteration of LINC00908, miR-483-5p and/or TSPYL5 determined by Western blot analysis, normalized to β-actin, *p < 0.05 vs. cells upon oe-NC treatment, ^#p < 0.05 vs. cells upon inhibitor NC treatment. The data were measurement data and expressed as the mean ± standard deviation. Unpaired t-test was used for data between two groups, while one-way ANOVA was used for data among multiple groups, followed by Tukey’s post hoc test. The cell experiment was repeated three times