Fig. 5

Silencing of miR-483-5p or overexpression of TSPYL5 exerts inhibitory effects on PCa cell proliferation, migration, and invasion, while stimulating apoptosis. LNCaP cells were treated with the miR-483-5p inhibitor, oe-TSPYL5, or miR-483-5p inhibitor and si-TSPYL5. a The effect of miR-483-5p and TSPYL5 on cell proliferation in each group detected by EdU assay (×200). b The protein expression of MMP-2 and MMP-9 measured by Western blot analysis, normalized to β-actin. c Cell invasion detected by Transwell assay (×200). d Cell migration detected by scratch test. e Cell apoptosis was detected by flow cytometry. f The cell cycle distribution measured by flow cytometry. *p < 0.05 vs. cells treated with inhibitor NC, and ^#p < 0.05 vs. cells treated with oe-NC. The results were measurement data and expressed as mean ± standard deviation. One-way ANOVA was used for data among multiple groups, followed by Tukey’s post hoc test. The cell experiment was repeated three times