Fig. 3

Knockdown of ENO1 suppressed the hyperglycemia-induced GC malignant phenotype in AGS and MGC803 cells. a, b Western blot analysis and qRT-PCR were performed to verify the transfection efficiency of ENO1. c CCK-8 assays were used to evaluate the effect of ENO1 knockdown on cell proliferation. d, e The effect of ENO1 silencing on migration was examined by wound healing and transwell migration assays. f Transwell invasion assays were used to assess the invasive ability of GC cells transfected with siENO1 in the normal and high glucose groups. *P < 0.05, **P < 0.01, ***P < 0.001