Fig. 2

Inhibited lncRNA-CIR restrains proliferation of MG63 cells. a The colony formation ability of MG63 cells was determined by colony formation assay; b Proliferation of MG63 cells was determined by EdU assay (50 μm); c Viability of MG63 cells was determined by CCK-8 assay; d Protein expression of PCNA and Ki67 in MG63 cells was evaluated by Western blot analysis; e Protein bands of PCNA and Ki67 in MG63 cells; *P < 0.05 vs the sh-NC group, ⁺P < 0.05 vs the oe-NC group. The measurement data conforming to the normal distribution were expressed as mean ± standard deviation, one-way ANOVA was used for comparisons among multiple groups and Tukey’s post hoc test was used for pairwise comparisons after one-way ANOVA. The experiment was repeated for 3 times