Fig. 4
From: UBQLN4 promotes progression of HCC via activating wnt-β-catenin pathway and is regulated by miR-370
Suppression of UBQLN4 inhibits proliferation and invasion of HCC cells in vitro. a Protein level of UBQLN4 between normal hepatocytes cell lines and HCC cell lines was compared. b Expression of UBQLN4 in Hep-G2 and SMMC-7721 cells was detected through immunofluorescence. c Transfect efficiency of UBQLN4 shRNA in Hep-G2 and SMMC-7721 cells was verified in protein level. d Cell viability of Hep-G2 and SMMC-7721 cells under treatment of UBQLN4 shRNA and negative control in 24 h, 48 h and 72 h. e, f Cell proliferation rate and tumor formation ability of Hep-G2 and SMMC-7721 cells under treatment of UBQLN4 shRNA and negative control. g Invasion ability of HCC cells with UBQLN4 shRNA and negative control was compared in vitro. Mean ± SD, *p < 0.05, **p < 0.01