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Fig. 4 | Cancer Cell International

Fig. 4

From: TSPAN9 suppresses the chemosensitivity of gastric cancer to 5-fluorouracil by promoting autophagy

Fig. 4

TSPAN9 affects the sensitivity of gastric cancer cells to 5-FU by mediating autophagy. a AGS cells were treated with 3-MA (a kind of autophagy inhibitor) and transfected with TSPAN9 plasmids prior to the addition of 100 µM 5-FU for 24 h. Cell viability was assessed by the CCK-8 assay. b AGS/R cells were treated with 3-MA and transfected with 100 µM 5-FU for 24 h. Cell viability was assessed by the CCK-8 assay. c AGS cells transfected with a TSPAN9-overexpressing vector and AGS/R cells treated with 3-MA were subjected to immunoblotting with the indicated antibodies. d MGC803 cells were treated with 3-MA and TSPAN9 plasmids and then treated with 100 µM 5-FU for 24 h. Cell viability was assessed by the CCK-8 assay. e MGC803/R cells were transfected with 3-MA and then treated with 100 µM 5-FU for 24 h. Cell viability was assessed by the CCK-8 assay. f MGC803 cells transfected with a TSPAN9-overexpressing vector and MGC803/R cells treated with 3-MA were subjected to immunoblotting with the indicated antibodies. *p < 0.05, **p < 0.01; Student’s t test

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