Fig. 6From: TSPAN9 suppresses the chemosensitivity of gastric cancer to 5-fluorouracil by promoting autophagyTSPAN9 inhibits PI3K–Akt–mTOR signaling by binding to PIK3R3 (p55). a Lysates from AGS/R cells stimulated with 5-FU were subjected to immunoprecipitation with anti-TSPAN9 followed by immunoblotting with anti-p55. b The endogenous TSPAN9 complex was analyzed by LC–MS–MS. c A schematic diagram of the TSPAN9 phosphorylation site. d AGS cells were transfected with TSPAN9 (WT) or TSPAN9 (Y153D). Lysates were subjected to immunoprecipitation with antibodies targeting p55 and TSPAN9. e AGS cells were transfected with either TSPAN9 (WT) or TSPAN9 (Y153D) plasmids and treated with 100 µM 5-FU for 24 h. Cell viability was assessed by the CCK-8 assay. f AGS cells were transfected with either TSPAN9 (WT) or TSPAN9 (Y153D) plasmids and subjected to immunoblotting for LC3 and P62Back to article page