Table 1 Summary of microsatellite instability detection methods
From: Microsatellite instability: a review of what the oncologist should know
Detection method | Characteristics | Test items | Accuracy | Refs. |
|---|---|---|---|---|
NGS | Accurate results were obtained from a small amount of sample | Nearly 100 MS loci | IMPACTâ„¢: 92% F1CDx: 94.6% | Hempelmann et al. [6] |
Fluorescent multiplex PCR and CE | Only MSI results are obtained MSI analysis system is based on this method | 5 MS sites: BAT-26, NR-21, BAT-25, MONO-27 and NR-24 | Gold standard, 100% | Arulananda et al. [11] |
IHC | Wide application and strong practicability, but only get the MMR results | The MMR protein: hMLH1, hPMS2, hMSH2, hMSH6 | 89–95% | Cheah et al. [12] |
smMIPs | Accurate and no matching of normal materials are required for certain diseases: colorectal cancer, prostate cancer, endometrial cancer | DNA from tumor tissue | 95.80% | Waalkes et al. [17] |