Fig. 4

AHNAK is a target gene of miR-93-5p. a Heat map of differentially expressed mRNAs in the GSE2685 expression dataset. Abscissa indicates differential p value, and ordinate indicates logFC. Each small dot in the Figure indicates a gene, among them red indicates a gene with significantly up-regulated expression in GC, green indicates a gene with significantly down-regulated expression in GC and black indicates genes which aren’t differentially expressed. b The intersection of miR-93-5p targeting gene prediction and down-regulated gene in the GSE2685 expression dataset. The left circle indicates the prediction of the target genes in TargetScan database, the right circle indicates the down-regulated genes in the GSE2685 expression dataset, and the middle part indicates the intersection. c The binding sites of miR-93-5p to AHNAK 3′UTR and OPCML 3′UTR. d Dual luciferase reporter gene assay to verify the targeting relationship between miR-93-5p and AHNAK. e Western blot analysis to determine the expression of AHNAK in immortalized human gastric mucosal epithelial cell line and GC cell lines. f Western blot analysis to investigate the effect of miR-93-5p on AHNAK expression. Data were measurement data and expressed as mean ± standard deviation. One-way analysis of variance was used for comparison among multi-groups. The cell experiment was repeated three times. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001