Fig. 5

Up-regulation of AHNAK inhibits HGC-27 cell migration, invasion and EMT. a Transwell assay to investigate the effect of AHNAK on HGC-27 cell invasion and migration (×200). Blank group: HGC-27 cells without transfection of any plasmids; Empty vector group: HGC-27 cells transfected with empty vector; AHNAK group: HGC-27 cells transfected with AHNAK overexpression plasmid. b Quantitative analysis for the role of overexpressed AHNAK in the HGC-27 cell migration and invasion. c Western blot analysis of EMT markers E-cadherin, Vimentin and Snail expression after overexpression of AHNAK. d Transwell assay of effect of miR-93-5p mimic and overexpressed AHNAK in HGC-27 cell invasion and migration (×200). Mimic NC + empty vector group: HGC-27 cells co-transfected with mimic NC and empty vector; miR-93-5p mimic + empty vector group: HGC-27 cells co-transfected with miR-93-5p mimic and empty vector; miR-93-5p mimic + AHNAK group: HGC-27 cells co-transfected with co-transfected with miR-93-5p mimic and AHNAK. e Quantitative analysis for the role of overexpressed AHNAK in the HGC-27 cell migration and invasive. f western blot analysis of EMT markers E-cadherin, Vimentin and Snail expression after overexpression of miR-93-5p and AHNAK. Data were measurement data and expressed as mean ± standard deviation. One-way analysis of variance was used for comparison among multi-groups. The cell experiment was repeated three times. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001