Fig. 2
TUG1 acted as a molecular sponge for miR-216b-5p. a The binding sites of TUG1 and miR-216b-5p were predicted by starBase v2.0. b, c Relative luciferase activity of Hep3B and Huh7 cells co-transfected TUG1-WT or TUG1-MUT with miR-216b-5p or miR-NC was checked by dual-luciferase reporter assay. d, e MiR-216b-5p expression in Hep3B and Huh7 cells transfected with TUG1, NC, si-TUG1 or si-NC was measured by qRT-PCR. f Statistical correlations between the expression levels of TUG1 and miR-216b-5p were analyzed by Spearman’s test in SPSS 20.0 software (R2 = 0.7447, P < 0.0001). *P < 0.05