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Fig. 3 | Cancer Cell International

Fig. 3

From: Long non-coding RNA TUG1 promotes cell progression in hepatocellular carcinoma via regulating miR-216b-5p/DLX2 axis

Fig. 3

The effect of interference with TUG1 on HCC cells could be restored by anti-miR-216b-5p. a, b The TUG1 expression in Hep3B and Huh7 cells transfected with si-NC, si-TUG1#1 or si-TUG1#2 was tested by qRT-PCR. ce The proliferation and cell cycle of Hep3B and Huh7 cells transfected with control, si-NC, si-TUG1#1, si-TUG1#1 + anti-miR-NC or si-TUG1#1 + anti-miR-216b-5p were detected by MTT and Flow cytometry. f, g The protein levels of CyclinD1 and CDK4 were checked by western blot. h The apoptosis of transfected Hep3B and Huh7 cells was determined by Flow cytometry. f, g Western blot assay was used to measure the levels of PARP, cleaved PARP and cleaved caspase-3. *P < 0.05

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