Fig. 1

LncRNA GAS5 is poorly expressed in melanoma tissues and cells and inhibits oxidative stress in melanoma. A, RT-qPCR assay of lncRNA GAS5 expression in melanoma tissues and adjacent normal tissues. B, Survival time analysis by Kaplan–Meier method (n = 75). C, RT-qPCR screening of the cell line with the lowest expression of lncRNA GAS5. D, The FISH assay of subcellular localization of lncRNA GAS5 (200 ×). E, CCK-8 assay of A375 and PIG1 cell viability. F, Flow cytometric analysis of A375 and PIG1 cell apoptosis. G, Western blot analysis of protein expression of MDA5, IRE1α, and SOD-1 in A375 and PIG1 cells. H, ELISA analysis of ROS content in A375 and PIG1 cells. *p < 0.05, compared with the adjacent normal tissues or PIG1 cell. The above measurement data are expressed as mean ± standard deviation. The Paired t-test is adopted to analyze the data of melanoma tissues and adjacent normal tissues, and the unpaired t-test is used for comparison of the other data between two groups. Data among multiple groups are analyzed by one-way ANOVA, followed by Tukey’s post hoc test. The statistical analysis concerning time-based measurements within each group is realized using ANOVA of repeated measurements, followed by a Bonferroni’s post hoc test. Pearson correlation analysis is performed for correlation analysis. Kaplan–Meier analysis is used for survival analysis