Fig. 2

EZH2 overexpression accelerates oxidative stress in melanoma cells by targeting CDKN1C. a, b, RT-qPCR assay of mRNA expression of EZH2 (a) and CDKN1C (b) in melanoma tissues and adjacent normal tissues. c, d, Western blot assay of protein expression of EZH (c) and CDKN1C (d) in melanoma tissues and adjacent normal tissues. e Survival time analysis by Kaplan–Meier method (n = 75). f Correlation analysis of CDKN1C expression and EZH2 expression. G, Dual-luciferase reporter gene assay of the relationship between EZH2 and CDKN1C. *p < 0.05, compared with the adjacent normal tissues or cells transduced with oe-E-NC. The above measurement data are expressed as mean ± standard deviation. The Paired t-test is adopted to analyze the data of melanoma tissues and adjacent normal tissues, and the unpaired t-test is used for other data comparisons between two groups. Data among multiple groups are analyzed by one-way ANOVA, followed by Tukey’s post hoc test