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Fig. 4 | Cancer Cell International

Fig. 4

From: Long non-coding RNA GAS5 accelerates oxidative stress in melanoma cells by rescuing EZH2-mediated CDKN1C downregulation

Fig. 4

EZH2 overexpression diminishes CDKN1C expression by facilitating H3K27 trimethylation to attenuating oxidative stress in melanoma. a RT-qPCR assay of mRNA expression of EZH2 and CDKN1C. b Western blot assay of protein expression of EZH2, H3K27me3 and CDKN1C. c ChIP assay of EZH2 and H3K27me3 enrichment in CDKN1C promoter region. d Western blot assay of protein expression of MDA5, IRE1α and SOD-1. E, ELISA analysis of ROS content. F, CCK-8 assay of cell viability. G, Flow cytometric analysis of cell apoptosis. *p < 0.05, compared with the oe-E-NC group and the sh-E-NC group. The above measurement data are expressed as mean ± standard deviation. The unpaired t-test is used for comparison of the data between two groups. The statistical analysis concerning time-based measurements within each group is realized using ANOVA of repeated measurements, followed by a Bonferroni’s post hoc test

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