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Fig. 6 | Cancer Cell International

Fig. 6

From: Long non-coding RNA GAS5 accelerates oxidative stress in melanoma cells by rescuing EZH2-mediated CDKN1C downregulation

Fig. 6

UNC1999 inhibits H3K27 trimethylation and upregulates CDKN1C expression to accelerate oxidative stress in melanoma. A375 cells were treated with sh-GAS5 in the presence of UNC1999 or DMSO. a RT-qPCR assay of lncRNA GAS5 expression and mRNA expression of EZH2 and CDKN1C. b Western blot assay of the protein expression of EZH2, H3K27me3 and CDKN1C. c Western blot assay of the protein expression of MDA5, IRE1α and SOD-1. d ELISA analysis of the content of ROS. E, CCK-8 assay of cell viability. F, Flow cytometric analysis of cell apoptosis. *p < 0.05, compared with the cells transduced with sh-GAS5 + DMSO. The above measurement data are expressed as mean ± standard deviation. The unpaired t-test is used for comparison of the data between two groups. The statistical analysis concerning time-based measurements within each group is realized using ANOVA of repeated measurements, followed by a Bonferroni’s post hoc test

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