Fig. 5

The influences of circ_0008035 silencing on GC cell proliferation, apoptosis and ferroptosis were weakened by miR-599 knockdown. HGC-27 and AGS cells were assigned to si-NC, si-circ_0008035, si-circ_0008035 + anti-miR-NC and si-circ_0008035 + anti-miR-599 groups. a, b The expression of miR-599 in HGC-27 and AGS cells was determined by qRT-PCR assay. c, d The proliferation of HGC-27 and AGS cells was analyzed by MTT assay. e, f The protein levels of cyclin D1 and PCNA were examined by western blot assay. g, h The apoptosis of HGC-27 and AGS cells was measured through flow cytometry analysis. i, j Cell survival rate was assessed by MTT assay after HGC-27 and AGS cells were treated with erastin or RSL3. *P < 0.05