Fig. 6

Circ_0008035 interacted with miR-599 to promote iron accumulation, lipid peroxidation and reduce mitochondrial membrane potential in the process of ferroptosis in GC cells. Si-NC, si-circ_0008035, si-circ_0008035 + anti-miR-NC or si-circ_0008035 + anti-miR-599 was transfected into HGC-27 and AGS cells and then these cells were treated with or without erastin or RSL3. a, b Total iron level, c, d Fe²⁺ accumulation, e, f MDA level, g, h lipid ROS level, i, j mitochondrial superoxide concentration and k, l mitochondrial membrane potential in HGC-27 and AGS cells were examined by specific kits. *P < 0.05