Fig. 7

Knockdown of circ_0008035 decreased EIF4A1 expression by targeting miR-599 in GC cells. a The potential binding sites between miR-599 and EIF4A1 3′ UTR were predicted by starBase 2.0. b, c The association between miR-599 and EIF4A1 was verified by dual-luciferase reporter assay. d, e The protein level of EIF4A1 in GC tissues, cells and corresponding normal tissues and cells was determined using western blot assay. f, g The protein level of EIF4A1 in HGC-27 and AGS cells transfected with miR-NC or miR-599 was measured by western blot assay. h, i HGC-27 and AGS cells were transfected with si-NC, si-circ_0008035, si-circ_0008035 + anti-miR-NC or si-circ_0008035 + anti-miR-599 and then the protein expression of EIF4A1 was examined by western blot assay. *P < 0.05