Fig. 2

Impact of circPITX1 knockdown on glycolysis and radioresistance of glioma cells. a–h U251 and LN229 cells were transfected with si-circPITX1 or si-NC. a QRT-PCR assay for the relative expression of circPITX1 in transfected glioma cells. b, c MTT assay for the viability of transfected glioma cells. d–f The glucose consumption, lactate production and ATP level in transfected U251 and LN229 cells. g, h Western blot assay for the levels of HIF-1α, HK2 and LDH-A in transfected U251 and LN229 cells. i, j Colony formation assay for the survival rate of U251 and LN229 cells transfected with si-circPITX1 or si-NC under X-ray radiation treatment (0 Gy, 2 Gy, 4 Gy, 6 Gy and 8 Gy). k, l Colony formation assay for the colony formation rate of U251 and LN229 cells transfected with si-circPITX1 or si-NC treated with 6 Gy of X-rays or not. *P < 0.05