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Fig. 3 | Cancer Cell International

Fig. 3

From: Circular RNA circPITX1 knockdown inhibits glycolysis to enhance radiosensitivity of glioma cells by miR-329-3p/NEK2 axis

Fig. 3

MiR-329-3p was a target of circPITX1. a The binding sites between circPITX1 and miR-329-3p, as well as the mutant. b, c Dual-luciferase reporter assay for the luciferase activity in glioma U251 and LN229 cells cotransfected with circPITX1-WT or circPITX1-MUT and miR-329-3p or miR-NC. d, e QRT-PCR assay for the relative expression of miR-329-3p in glioma tissues and normal tissues, as well as in glioma U251 and LN229 cells and NHA. f Pearson correlation analysis for the correlation between the expression of circPITX1 and miR-329-3p in glioma tissues. R = − 0.574, P < 0.0001. g QRT-PCR assay for the relative expression of miR-329-3p in U251 and LN229 cells transfected with si-circPITX1 or si-NC. h QRT-PCR assay for the relative expression of miR-329-3p in U251 and LN229 cells transfected with pcDNA-circPITX1 or pcDNA-NC. *P < 0.05

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