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Fig. 4 | Cancer Cell International

Fig. 4

From: Circular RNA circPITX1 knockdown inhibits glycolysis to enhance radiosensitivity of glioma cells by miR-329-3p/NEK2 axis

Fig. 4

CircPITX1 knockdown exerted inhibitory effects on the glycolysis and radioresistance of glioma cells by sponging miR-329-3p. a–h U251 and LN229 cells were transfected with si-NC, si-circPITX1, si-circPITX1 + anti-miR-NC or si-circPITX1 + anti-miR-329-3p. a QRT-PCR assay for the relative expression of miR-329-3p in transfected glioma cells. b, c MTT assay for the viability of transfected glioma cells. d–f The glucose consumption, lactate production and ATP level in transfected U251 and LN229 cells. g, h Western blot assay for the levels of HIF-1α, HK2 and LDH-A in transfected U251 and LN229 cells. i, j Colony formation assay for the survival rate of U251 and LN229 cells transfected with si-NC, si-circPITX1, si-circPITX1 + anti-miR-NC or si-circPITX1 + anti-miR-329-3p under X-ray radiation treatment (0 Gy, 2 Gy, 4 Gy, 6 Gy and 8 Gy). k, l Colony formation assay for the colony formation rate of U251 and LN229 cells transfected with si-NC, si-circPITX1, si-circPITX1 + anti-miR-NC or si-circPITX1 + anti-miR-329-3p treated with 6 Gy of X-rays or not. *P < 0.05

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