Fig. 8

Glycolysis inhibitor 2-deoxy-d-glucose (2-DG) treatment weakened the elevated effects of circPITX1 on glycolysis. a Colony formation assay for the survival rate of U251 and LN229 cells treated with 2-DG or not (Control). b–f U251 and LN229 cells treated with pcDNA-NC, pcDNA-circPITX1 or pcDNA-circPITX1 + 2-DG. b–d The glucose consumption, lactate production and ATP level in treated U251 and LN229 cells. e, f Western blot assay for the levels of HIF-1α, HK2 and LDH-A in treated U251 and LN229 cells. g, h Colony formation assay for the survival rate of U251 and LN229 cells treated with pcDNA-NC, pcDNA-circPITX1 or pcDNA-circPITX1 + 2-DG under X-ray radiation treatment (0 Gy, 2 Gy, 4 Gy, 6 Gy and 8 Gy). i, j Colony formation assay for the colony formation rate of U251 and LN229 cells treated with pcDNA-NC, pcDNA-circPITX1 or pcDNA-circPITX1 + 2-DG with 6 Gy of X-rays treatment or not. *P < 0.05