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Fig. 5 | Cancer Cell International

Fig. 5

From: Down-regulation of LINC00472 promotes osteosarcoma tumorigenesis by reducing FOXO1 expressions via miR-300

Fig. 5

FOXO1 attenuated the effects of miR-300 mimic in OS cells. a The expressions of FOXO1 mRNA levels in MG63 and U2OS cells had transfection with vector and pcDNA-FOXO1. b CCK8 assays. c Colony formation experiment results and the cell numbers. d Transwell migration and invasion assays, and the cell numbers. e Western blotting for the expression of PCNA, Ki67, and GAPDH in MG63 and U2OS cells transfected by control, mi-300 mimic, or mi-300 mimic mi-300 mimic + pcDA-foxo1. *P < 0.001, **P < 0.05, n = 5

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