Fig. 5
Knockdown of ADAM10 inhibited tumorigenicity of osteosarcoma cells. A single cell suspension of 0.5 mL containing 5 × 106 of transfected cells was subcutaneously injected into each mouse at the left under-axillary. Tumor volume was measured every 3 days since day 7 after cell injection (a). Tumors were weight (b) and taken photo up 28 days after cell injection (c). The expressions of ADAM10, E-cadherin, Cyclin D1, c-Myc, and Survivin in osteosarcoma tissues were detected by western blot (d). The localization and expression of β-catenin were detected by immunofluorescence assay (e) and western blot (f) in the osteosarcoma tissues (*P < 0.05)