Fig. 2
From: CREB1-induced miR-1204 promoted malignant phenotype of glioblastoma through targeting NR3C2
MiR-1204 was transcriptionally activated by CREB1 in GBM. a The upregulation of CREB1 in GBM cells was confirmed by RT-qPCR. b The overexpression efficiency of CREB1 in A172 cells and the knockdown efficiency of CREB1 in U251 cells were confirmed by RT-qPCR. c MiR-1204 expression under the overexpression and knockdown of CREB1 in A172 and U251 cells were detected by RT-qPCR. d The DNA motif of CREB1 and the CREB1 binding site on miR-1204 promoter were obtained from miRGen. e Luciferase reporter assay was used to detect the impact of CREB1 on miR-1204 transcription. f ChIP assay was used to confirm the binding capacity between CREB1 and miR-1204 promoter. GAPDH was used as internal control for CREB1 expression detection, and U6 was used as internal control for miR-1204 expression detection. *P < 0.05, **P < 0.01