Fig. 3
From: CREB1-induced miR-1204 promoted malignant phenotype of glioblastoma through targeting NR3C2
MiR-1204 targeted NR3C2 to repress its expression in GBM. a Binding sequences between NR3C2 and miR-1204 were obtained from miRDB and the mutant sequences were designed. b Downregulation of NR3C2 in GBM samples was obtained from GEPIA. c Downregulation of NR3C2 in GBM cell lines was confirmed by RT-qPCR. d, e Luciferase reporter assay and RIP assay were used to detect the interaction between miR-1204 and NR3C2 mRNA. NS no significance. f NR3C2 expression under the overexpression and knockdown of miR-1204 was detected by RT-qPCR and western blot assays. GAPDH was used as internal control for NR3C2 expression detection, and U6 was used as internal control for miR-1204 expression detection.*P < 0.05, **P < 0.01, ***P < 0.001