Table 1 Related PCR primers and primers for plasmids
From: CREB1-induced miR-1204 promoted malignant phenotype of glioblastoma through targeting NR3C2
Gene name | PCR primers (5′-3′) |
|---|---|
miR-1204 | F:GUGGCCUGGUCUCCAUUACC R:CTCTACAGCTATATTGCCAGCCAC |
CREB1 | F:TGCAACATCATCTGCTCCCA R:CTGAATAACTGATGGCTGGGC |
NR3C2 | F:GAGAGCCCACATTGCTAGCA R:GCCCTGCTGGAATCAACTCT |
GAPDH | F:GAGAAGGCTGGGGCTCATTT R:AGTGATGGCATGGACTGTGG |
U6 | F:CTCGCTTCGGCAGCACA R:AACGCTTCACGAATTTGCGT |
Plasmid name | Related sequences (5′-3′) |
|---|---|
NC mimic | UCCCGGGGUGGUCUCCAUUAU |
miR-1204 mimic | UCGUGGCCUGGUCUCCAUUAU |
NC inhibitor | AUAAUGGAGACCACCCCGGGA |
miR-1204 inhibitor | AUAAUGGAGACCAGGCCACGA |
sh-NC | CCGCCAGAAATGCAAATTCTTATTCCTCGAGGAATAAGAATTTGCATTTCTGTTTTTG |
sh-CREB1#1 | CCGCCAACTATTGCAGAAAGTGAAGCTCGAGCTTCACTTTCTGCAATAGTTGTTTTTG |
sh-CREB1#2 | CCGCGAAAATTTTGAATGACTTATCCTCGAGGATAAGTCATTCAAAATTTTCTTTTTG |
sh¬NC | CCGCCAGAAACAACAAAAAGTAAAACTCGAGTTTTACTTTTTGTTGTTTCTGTTTTTG |
sh¬NR3C2#1 | CCGCGAATAATAGTCTTTATCATCCCTCGAGGGATGATAAAGACTATTATTCTTTTTG |
sh¬NR3C2#2 | CCGCGAAACACAGCTTACGTTGACACTCGAGTGTCAACGTAAGCTGTGTTTCTTTTTG |
pcDNA3.1/CREB1 | ATGACCATGGAATCTGGAGCCGAGAACCAGCAGAGTGGAGATGCAGCTGTAACAGAAGCTGAAAACCAAC AAATGACAGTTCAAGCCCAGCCACAGATTGCCACATTAGCCCAGGTATCTATGCCAGCAGCTCATGCAAC ATCATCTGCTCCCACCGTAACTCTAGTACAGCTGCCCAATGGGCAGACAGTTCAAGTCCATGGAGTCATT CAGGCGGCCCAGCCATCAGTTATTCAGTCTCCACAAGTCCAAACAGTTCAGATTTCAACTATTGCAGAAA GTGAAGATTCACAGGAGTCAGTGGATAGTGTAACTGATTCCCAAAAGCGAAGGGAAATTCTTTCAAGGAG GCCTTCCTACAGGAAAATTTTGAATGACTTATCTTCTGATGCACCAGGAGTGCCAAGGATTGAAGAAGAG AAGTCTGAAGAGGAGACTTCAGCACCTGCCATCACCACTGTAACGGTGCCAACTCCAATTTACCAAACTA GCAGTGGACAGTATATTGCCATTACCCAGGGAGGAGCAATACAGCTGGCTAACAATGGTACCGATGGGGT ACAGGGCCTGCAAACATTAACCATGACCAATGCAGCAGCCACTCAGCCGGGTACTACCATTCTACAGTAT GCACAGACCACTGATGGACAGCAGATCTTAGTGCCCAGCAACCAAGTTGTTGTTCAAGCTGCCTCTGGAG ACGTACAAACATACCAGATTCGCACAGCACCCACTAGCACTATTGCCCCTGGAGTTGTTATGGCATCCTC CCCAGCACTTCCTACACAGCCTGCTGAAGAAGCAGCACGAAAGAGAGAGGTCCGTCTAATGAAGAACAGG GAAGCAGCTCGAGAGTGTCGTAGAAAGAAGAAAGAATATGTGAAATGTTTAGAAAACAGAGTGGCAGTGC TTGAAAATCAAAACAAGACATTGATTGAGGAGCTAAAAGCACTTAAGGACCTTTACTGCCACAAATCAGA T |