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Fig. 4 | Cancer Cell International

Fig. 4

From: Long non-coding RNA NEAT1/miR-338-3p axis impedes the progression of acute myeloid leukemia via regulating CREBRF

Fig. 4

Down-regulation of CREBRF abrogated the NEAT1-induced effects on AML cells. a, b QRT-PCR and Western blot were performed to measure the mRNA and protein expression of CREBRF in KG-1 and HL-60 cells transfected with NC, NEAT1, NEAT1 + si-NC or NEAT1 + si-CREBRF. c The colony formation ability of transfected cells was analyzed via colony formation assay. d, e MTT was used to measure the cell viability. f Flow cytometry was applied to determine the apoptosis rate. g, h Cleaved PARP and Cleaved caspase-3 levels in transfected AML cells were examined by Western blot. i, j Transwell assay was implemented for evaluating cell migration and invasion abilities. *P < 0.05

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