Fig. 6

NEAT1/miR-338-3p modulated cell growth, apoptosis, migration and invasion of AML cells by affecting CREBRF. a, b QRT-PCR and Western blot were performed to measure the CREBRF mRNA and protein levels in KG-1 and HL-60 cells transfected with NC, NEAT1, NEAT1 + miR-NC or NEAT1 + miR-338-3p. c–e Cell growth was assessed using colony formation assay (c) and MTT assay (d, e). f–h Cell apoptosis was evaluated through apoptosis rate by flow cytometry (f) and the detection of Cleaved PARP and Cleaved caspase-3 protein levels by Western blot (g, h). i, j The assessment of cell migration and invasion was implemented via transwell assay. *P < 0.05