Fig. 3

Combination of S109 and radiation results in cell cycle arrest and enhances radiation-induced DNA damage. The distribution of cell cycle after S109 and/or IR treatment and quantitative analyses were examined by flow cytometry in U87 cells a and C6 cells b. The data represent the mean ± SD from 3 independent experiments, *P < 0.05, **P < 0.01. c–f The expression levels of multiple cell-cycle- and apoptosis-associated proteins were assessed by western blot assay in time- and does-manner. g Lysates of S109-treated U87 cells were collected at 24 h after radiation (2 Gy) for Western blot analysis. The levels of γ-H2AX and H2AX were examined