Fig. 5

Combination of S109 and radiation reduces NF-κB transcriptional activation and promotes the nuclear accumulation of IκΒα. U87 and C6 cells were treated with S109 and/or IR. Lysates of cells were collected at 24 h after radiation for Western blot. The expression levels of CRM1, p-p65 and p-65 were assessed in a dose- and time-manner a–d. e The effects of S109 and/or IR treatment on the subcellular location of IκΒα. The cytoplasmic and nuclear protein extracts were used for immunoblotting with the indicated antibodies. f The transactivation ability of NF-κB were inhibited when combination of S109 and radiation. Relative NF-κB luciferase activity normalized with respect to corresponding renilla luciferase activity is shown. All the Data are presented as mean ± SD, *P < 0.05, **P < 0.01