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Fig. 6 | Cancer Cell International

Fig. 6

From: Novel monoclonal antibodies against thymidine kinase 1 and their potential use for the immunotargeting of lung, breast and colon cancer cells

Fig. 6

Evaluation of three antibody pairs for TK1 detection in sandwich ELISA. a Dose response curves and 4-point non-linear regression analysis of three antibody pairs. b The signal obtained from TK1 detection in serial dilutions with three antibody pairs is proportional to the concentration of antigen. c Optimization of capture and detection antibodies concentration ratios. d Spiking recovery assay. Assessment of the interference of human serum with the antibodies’ capacity to detect a known concentration of TK1. Recovery was around 100% with 10% serum dilution. e Specificity of the immunoassay to TK1. Using cell lysate from a high expressing TK1 cancer cell line and lysate from cells treated with siRNA TK1 the specificity of the immunoassay was evaluated. Signal from the siRNA TK1 knockdown cell lysate was significantly lower compared to the untreated cell lysate

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Cancer Cell International

ISSN: 1475-2867

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