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Fig. 7 | Cancer Cell International

Fig. 7

From: Novel monoclonal antibodies against thymidine kinase 1 and their potential use for the immunotargeting of lung, breast and colon cancer cells

Fig. 7

Detection of TK1 surface expression through flow cytometry. Four different cancer cell lines expressing membrane associated TK1 were stained with the custom TK1 antibodies. The binding capacities of the custom antibodies were compared to a commercial TK1 antibody. a The average percentage value of positive cells to TK1 from three independent flow cytometry runs across 4 cancer cell types having lung cancer cells the maximum percentage of TK1 positive cells (95.6%) followed by prostate (72.2%), colon (62.4%) and breast (49.1%). b Clones 3B4, 8G2, 10E8 and 7H2 showed consistent binding for detection of TK1 surface expression in lung, prostate, breast and colon cancer cell lines. In the case of H460 cells which had the highest expression levels of TK1 on the cell membrane antibodies from epitope 1 and 6 showed binding consistently. Although 5F7G11 did not showed high specificity to TK1 when tested with a TK1 knockdown the antibody showed consistent binding to the surface of all cancer cell lines were analyzed. In addition, antibody 8F12 showed consistent binding too but, did not produce any signal in indirect ELISA

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