Fig. 2

MSC-AS1 played oncogenic role in NPC cells. a Images and quantification of invaded CNE-1 and 5-8F cells transfected with sh-NC, sh-MSC-AS1#1, or sh-MSC-AS1#2 was obtained through transwell assay (scale bar = 200 μm). (b–c) Western blot results and IF (scale bar = 50 μm) staining pictures showed E-cadherin and N-cadherin levels in CNE-1 and 5-8F cells under MSC-AS1 silence. d Morphology of cells transfected with sh-NC, sh-MSC-AS1#1/2 was shown. Scale bar = 200 μm. e Overexpression of MSC-AS1 with pcDNA3.1/MSC-AS1 vector. f–g Colony formation and EdU assays (scale bar = 200 μm) assessed the proliferation in MSC-AS1-overexpressed NPC cells. h Transwell invasion assay (scale bar = 200 μm) was conducted to test cell invasion after overexpression of MSC-AS1. (i–j) EMT markers were assessed with western blot analysis and IF (scale bar = 50 μm) in pcDNA3.1 and pcDNA3.1/MSC-AS1 groups. k Morphology of SNUE1 cell was observed with the transfection of pcDNA3.1 and pcDNA3.1/MSC-AS1. Scale bar = 200 μm. ^**P < 0.01