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Fig. 3 | Cancer Cell International

Fig. 3

From: LncRNA MSC-AS1 aggravates nasopharyngeal carcinoma progression by targeting miR-524-5p/nuclear receptor subfamily 4 group A member 2 (NR4A2)

Fig. 3

MSC-AS1 sponged miR-524-5p in NPC cells. a Subcellular fractionation and FISH (scale bar = 50 μm) showed MSC-AS1 level in cytoplasm and nucleus of CNE-1 and 5-8F cells. b Heatmap showed the level of 54 predicted miRNAs in 3 pairs of NPC tissues and adjacent non-tumor tissues. c RT-qPCR results of miR-524-5p level in NPC cells and nasopharyngeal epithelial cells. d RIP assay was used to determine the interaction between MSC-AS1 and miR-524-5p. e The miR-524-5p binding site on MSC-AS1 and designed mutated site were shown. f Luciferase reporter assay was applied to detect the interaction between miR-524-5p and MSC-AS1. g RT-qPCR results confirmed the overexpression efficiency of miR-524-5p in CNE-1 and 5-8F cells. h Expression of MSC-AS1 in CNE-1 and 5-8F cells under miR-524-5p overexpression was detected by RT-qPCR. i Expression of miR-524-5p in CNE-1 and 5-8F cells under MSC-AS1 silence was detected by RT-qPCR. j Pearson’s correlation analysis showed the negative expression correlation between MSC-AS1 and miR-524-5p in NPC tissues. **P < 0.01, ***P < 0.001

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