Fig. 4

MSC-AS1 induced NR4A2 level by targeting miR-524-5p in NPC cells. a The list and Venn pattern showed that PKN2, NR4A2, NEDD4, and SIK2 were potentially targeted by miR-524-5p as predicted by PITA, RNA22, miRmap and microT. b RNA pull down assay was used to examine the interaction of miR-524-5p with the predicted mRNAs. c RT-qPCR results of NR4A2 expression in NPC tissues and the paired adjacent non-tumor tissues. d Pearson’s correlation curve showed that NR4A2 was positively correlated with MSC-AS1 and negatively correlated with miR-524-5p in NPC tissues. e Expression of NR4A2 in NPC cells and nasopharyngeal epithelial cells. f The miR-524-5p binding site on NR4A2 and designed mutated site. Luciferase reporter assay was used to detect the interaction between miR-524-5p and NR4A2. g RIP assay was used to determine the interaction of miR-524-5p with MSC-AS1 and NR4A2. h RT-qPCR and western blot results of mRNA and protein levels of NR4A2 in NPC cells under miR-524-5p overexpression. i RT-qPCR and western blot results of mRNA and protein levels of NR4A2 in NPC cells with indicated transfections. ^**P < 0.01, ^***P < 0.001