Fig. 5

Knockdown of NR4A2 suppressed NPC cell growth and EMT process. a NR4A2 was silenced in two NPC cells with specific shRNAs. b–d Cell proliferation was monitored after indicated transfections through CCK-8 assay, colony formation assay and EdU assay (scale bar = 200 μm). e–f Caspase-3 activity and TUNEL-positive cells in NR4A2-downregulated group and control group were observed. Scale bar = 200 μm. g Levels of EMT-relevant proteins in NR4A2 silenced cells were monitored through western blot analysis. ^**P < 0.01