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Fig. 2 | Cancer Cell International

Fig. 2

From: Long non-coding RNA LINC01419 mediates miR-519a-3p/PDRG1 axis to promote cell progression in osteosarcoma

Fig. 2

LINC01419 sponges miR-519a-3p in OS. a–b FISH assay (scale bar = 50 μm) and subcellular fractionation assay were implemented to determine the location of LINC01419 in OS cells. c Four miRNAs that bound to LINC01419 were screened out from ENCORI database. d MS2-RIP assay was conducted to assess the binding of 4 mirnas to linc01419. e Expressions of miR-519a-3p and miR-519c-3p in OS cells were investigated via qRT-PCR. f Binding sites between LINC01419 and miR-519a-3p were predicted by ENCORI database. g RNA pull down assay was implemented to evaluate the interaction between LINC01419 and miR-519a-3p in OS cells. h Overexpression efficiency of miR-519a-3p in U2OS and Saos-2 cells was examined by qRT-PCR. i Luciferase reporter assay verified the binding of LINC01419 to miR-519a-3p in U2OS and Saos-2 cells. **P < 0.01

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