Fig. 3

PDRG1 is targeted by miR-519a-3p and promotes OS cell progression. a Venn diagram was generated to show the potential mRNAs targeted by miR-519a-3p. b The expressions of mRNAs regulated by LINC01419 or miR-519a-3p were shown by qRT-PCR. c qRT-PCR analysis was conducted to estimate the expression of PDRG1 in OS cells. d Binding site between PDRG1 and miR-519a-3p was presented. e–f. RNA pull down assay and luciferase reporter assay assessed the combination of PDRG1 with miR-519a-3p. g Inhibition efficiency of PDRG1 was detected by qRT-PCR. h–i. Effect of PDRG1 silencing on U2OS and Saos-2 cell proliferation was evaluated by colony formation and EdU assays. j–k. The apoptosis of U2OS and Saos-2 cells was examined by JC-1 and TUNEL assays with or without PDRG1 silence. l–m. The migration and invasion in indicated cells were tested by transwell assay. n. IF assay (scale bar = 50 μm) was performed to investigate EMT process in U2OS and Saos-2 cells upon PDRG1 knockdown. ^**P < 0.01