Fig. 4

LINC01419 contributes to malignant phenotypes of OS cells through targeting miR-519a-3p/PDRG1 axis. a Effect of LINC01419 depletion or miR-519a-3p upregulation on PDRG1 expression was estimated by qRT-PCR. b–c qRT-PCR analysis was used to determine the transfection efficiency of miR-519a-3p inhibitor or pcDNA3.1-PDRG1. d–e. Cell proliferation in each group was evaluated by colony formation and EdU assays. f–g. JC-1 and TUNEL assays were carried out to assess cell apoptosis in each group. h–i. The motility of cells under indicated transfections was detected by transwell assay. j–k. IF assay (scale bar = 50 μm) and western blot analysis were employed to test changes on EMT process in each group. ^**P < 0.01