Table 1 Sequences of primers used in qRT-PCR and of plasmids applied in cell transfection
Genes | Sequences of primers (5′–3′) |
|---|---|
LINC01419 | F: AGAACTGAGGTCCACTTTCTGG |
R: GGTCCTTTGTCTGCAACGA | |
miR-519a-3p | F: AAAGTGCATCCTTTTAGAGTGTGG |
R: CTCTACAGCTATATTGCCAGCCAC | |
miR-519c-3p | F: AAAGTGCATCTTTTTAGAGGATCG |
R: CTCTACAGCTATATTGCCAGCCAC | |
miR-519b-3p | F: AAAGTGCATCCTTTTAGAGGTTCC |
R: CTCTACAGCTATATTGCCAGCCAC | |
miR-3121-3p | F: TAAATAGAGTAGGCAAAGGACAGCC |
R: CTCTACAGCTATATTGCCAGCCAC | |
PDRG1 | F: TGAGCCACAAAGCACCAA |
R: TCACATGGACCCTGAGCA | |
GAPDH | F: CATCCTGGGCTACACTGAGC |
R: AGTGGTCGTTGAGGGCAA | |
U6 | F: AAAGCAAATCATCGGACGACC |
R: GTACAACACATTGTTTCCTCGGA |
shRNAs/pcDNA3.1 | Sequences (5′-3′) |
 sh-NC | CCGGCACTAATGTCTGCGTCAATGTCTCGAGACATTGACGCAGACATTAGTGTTTTTG |
 sh-LINC01419#1 | CCGGTGCATAGATTCATTAAGAATTCTCGAGAATTCTTAATGAATCTATGCATTTTTG |
 sh-LINC01419#2 | CCGGATCTATGAAGCCAAACCTAATCTCGAGATTAGGTTTGGCTTCATAGATTTTTTG |
 sh-NC | CCGGGTGACATAGTGCACAGTTGGACTCGAGTCCAACTGTGCACTATGTCACTTTTTG |
 sh-PDRG1#1 | CCGGCACCAAGATTCTGTTCTTCATCTCGAGATGAAGAACAGAATCTTGGTGTTTTTG |
 sh-PDRG1#2 | CCGGACCAAGATTCTGTTCTTCATTCTCGAGAATGAAGAACAGAATCTTGGTTTTTTG |
 pcDNA3.1-P DRG1 | ATGCTATCACCCGAGGCAGAGCGAGTGCTGCGGTACCTTGTAGAAGTGGAGGAGCTCGCCGAGGAGGTGCTGGCGGACAAGCGGCAGATTGTGGACCTGGACACTAAAAGGAATCAGAATCGAGAGGGCCTGAGGGCCCTGCAGAAGGATCTCAGCCTCTCTGAAGATGTGATGGTTTGCTTCGGGAACATGTTTATCAAGATGCCTCACCCTGAGACAAAGGAAATGATTGAAAAAGATCAAGATCATCTGGATAAAGAAATAGAAAAACTGCGGAAGCAACTTAAAGTGAAGGTCAACCGCCTTTTTGAGGCCCAAGGCAAACCGGAGCTGAAGGGTTTTAACTTGAACCCCCTCAACCAGGATGAGCTTAAAGCTCTCAAGGTCATCTTGAAAGGA |
Mimics/inhibitor | |
 NC mimics | AUUCACGUUCCUUUUAGAGUGU |
 miR-519a-3p mimics | AAAGUGCAUCCUUUUAGAGUGU |
 NC inhibitor | ACACUCUAAAAGGAACGUGAAU |
 miR-519a-3p inhibitor | ACACUCUAAAAGGAUGCACUUU |