Fig. 2

The expression and combination with its noncompetitive antibody of 7D12. a The EGFR-antibody complex with one antibody and another noncompetitive single domain antibody on Hela cells. b SDS-PAGE analysis of 7D12 after being carried out in E. coli. In SDS-PAGE imaging, lane M was protein marker, lane 1 was protein expression by non-induced E. coli, lane 2 was protein expression by induced E. coli, lane 3 was supernatant after E. coli disruption, lane 4 was sedimentation after E. coli disruption. In the western blotting analysis of 7D12 after purification, lane M was protein marker, lane 1 was protein after purification. c 40 nM 7D12 (red) was treated on Hela cells for 1 h, cell nuclei were dyed by Hoechst 33342 (blue). Bars, 10 μm. d 40 nM 7D12 (red) were treated on Hela cells for 10 min, cell nuclei were dyed by Hoechst 33342 (blue). Bars, 10 μm. e 20 nM Cetuximab (green) and 20 nM 7D12 (red) were treated on Hela cells for 10 min, cell nuclei were dyed by Hoechst 33342 (blue). Bars, 10 μm