Fig. 3

Noncompetitive antibody combination triggered high intensity internalization by macropinocytosis. a, b 20 nM Cetuximab and 20 nM H11 (green) were treated on Hela cells for 1 h after different inhibitors pre-incubated for 30 min. All data are mean ± SD. Bars, 10 μm. Statistical significance was calculated with a nonparametric Kruskal–Wallis test, in which a P < 0.0001 was considered significant. c 20 nM Cetuximab and 20 nM H11 (green) and 100 μg/mL Dextran or BSA (red) co-treated on Hela cells for 1 h before cells imaging. Bars, 10 μm. d 20 nM Cetuximab and 20 nM H11 were treated on Hela cells for 1 h after clathrin/caveolin was interfered by its siRNA. All data are mean ± SD. e Hela cell nuclei were dyed by Hoechst 33342 after different single antibody (40 nM) or combination of antibodies (20 nM each antibody) treating for 10 min. Arrowheads showed the cup-shaped macropinosomes. Bars, 5 μm