Fig. 4

Lattice complex assembled along F-actin on cell membrane. a Different single antibody (40 nM) or combination of antibodies (20 nM each antibody) was treated on Hela cells for 10 min or 1 h, cell nuclei were dyed by Hoechst 33342 (blue). Afterward, F-actin was stained with AlexaFluor 488 Phalloidin. Bars, 10 μm. b 20 nM Cetuximab and 20 nM H11 (red) were treated on Hela cells for 10 min, F-actin was stained with AlexaFluor 488 Phalloidin. Arrowheads showed the lattice complex. Bars, 10 μm. c Surface rendering of images from (b) was generated from Imaris software. Bars, 1 μm. d, e Different combination of antibodies (20 nM each antibody) or single antibody (40 nM) was treated on Hela cells for 10 min, F-actin was stained with AlexaFluor 488 Phalloidin. Surface rendering of images was generated from Imaris software. Bars above, 10 μm, bars below, 10 μm