Fig. 3

BSN-AS2 could bind with miR-654-3p in spinal OS. a Nuclear-cytoplasmic fractionation assay disclosed BSN-AS2 expression was enriched in cytoplasm. b qRT-PCR unveiled the expression of 6 miRNAs in hFOB, U2OS and Saos-2 cells. c qRT-PCR manifested the expression of miR-654-3p in miR-654-3p mimics transfected cells. d qRT-PCR testified the expression of miR-654-3p when downregulating BSN-AS2 or upregulating E2F1 as well as the expression of BSN-AS2 when overexpressing miR-654-3p. e RIP assay showed the enrichment of BNS-AS2 and miR-654-3p in anti-Ago2 or anti-IgG group. f RNA pull down assay revealed BSN-AS2 could bind with miR-654-3p. g The expression of miR-654-3p in spinal OS tissues and adjacent-normal tissues was measured by qRT-PCR (left); Pearson correlation analysis illustrated the correlation between miR-654-3p and BSN-AS2 (right). h The putative binding sites between BSN-AS2 and miR-654-3p. i Luciferase reporter assay uncovered BSN-AS2 could bind with miR-654-3p. j, k CCK-8 and colony formation assays measured cell proliferation in response to miR-654-3p overexpression. l, m Flow cytometry analysis and TUNEL assay detected cell apoptosis in miR-654-3p mimics transfected cells. n, o Transwell assays illustrated cell migration and invasion when overexpressing miR-654-3p. ^**P < 0.01