Fig. 5

MiR-654-3p targeted SYTL2 to regulate spinal OS progression. a StarBase showed SYTL2 and CLN8 were potentially targeted by miR-654-3p. b qRT-PCR indicated the expression of SYTL2 and CLN8 in spinal OS cells and hFOB cells. c, d qRT-PCR and western blot assays uncovered the mRNA and protein expressions of STYL2. e RIP assay manifested the enrichment of BSN-AS2, miR-654-3p and SYTL2 in anti-Ago2 group. f RNA pull down showed miR-654-3p could bind with SYTL2. g The expression of SYTL2 in spinal OS tissues and adjacent-normal tissues was measured by qRT-PCR (left); Pearson correlation analysis illustrated the correlation between SYTL2 and miR-654-3p/BSN-AS2 (middle and right). h The potential binding site for miR-654-3p and SYTL2 was exhibited. i Luciferase reporter assay illustrated miR-654-3p could target SYTL2 in spinal OS. j qRT-PCR and western blot assays detected the mRNA and protein expressions of SYTL2. k, l CCK-8 and colony formation assays showed cell proliferation when inhibiting SYTL2. m, n Flow cytometry analysis and TUNEL assay determined cell apoptosis in SYTL2 silenced cells. o, p Transwell assay demonstrated cell migration and invasion in response to SYTL2 attenuation. ^**P < 0.01